with Plant RNA Reagent. Accidental thawing may result in RNA degradation. Procedure 1. ®Add 0.5 mL cold (4°C) PureLink Plant RNA Reagent to ≤0.1 gram of frozen, ground plant tissue. Mix by briefly vortexing or by flicking the bottom of the tube until the sample is thoroughly resuspended. 2. Incubate the tube for 5 minutes at room temperature.
Aug 17, 2009· Shake plant tissues at 35 rpm in the dark at room temperature for 16-18 h. Collect the released protoplasts into 50 ml Falcon tube by sieving through 8 layers of the cheese cloth, pre-wet in W5 Solution. Sieve protoplasts from the cheese-cloth one more time by .
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Spin Falcon tubes in a cooled centrifuge for 3 min at 3000 rpm to pellet the beads and transfer the supernatant to a new Falcon tube. Be careful not to carry over beads.
3. Transfer to Falcon tube and agitate for 30 min at 4C. 4. Centrifuge 10 min at 5000 g, 4C. 5. Remove phenol phase (should be top phase) and back-extract the aqueous phase with 2.5 mL + 2.5 mL of extraction media and phenol by vortexing. Centrifuge and combine with first extraction. 6.
Filter Attach pluriStrainer® to a sterile 50 ml centrifuge tube. Then, add sample material onto the strainer and filter sample Then, add sample material onto the strainer and filter sample Reversible To obtain the larger fraction, take off the pluriStrainer®, turn it upside down onto another 50 .
Collect pollen by centrifugation using 50 ml falcon tubes. (450 x g, 5 min, 4 °C). Repeat this step until all the pollen suspension is finished. Transfer pollen pellet into a 1.5 ml centrifuge tube. You can stop here by storing pollen at -80 °C, or proceed to the RNA isolation steps. Homogenization: Put .
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Pikaard lab plant RNA/DNA isolation protocol. Add ~2 g plant material to grind (as little as 0.25g works fine also without modifying the protocol). Grind plant material to a fine powder adding liquid nitrogen as necessary to avoid thaw. Transfer plant powder to a 15 ml snap cap Falcon tube containing 4 ml of RNA/DNA extraction buffer and vortex immediately.
TRAP Procedure – Isolation by immunoprecipitation of polysomes from transgenic plants expressing FLAG-tagged RPL18 ... - The plant material must be harvested directly into liquid nitrogen, ground to a fine powder ... - Centrifuge the 15 mL Falcon .
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Sterilin single use centrifuge tubes are the reliable and cost effective tool for microbiological or cell culture procedures and general centrifugation applications. The new superior quality 15ml centrifuge tube was specifically developed for high speed centrifugation up to 12,000 x g and has crisp, clear, easy to read graduations.
- Centrifuge the 15 mL Falcon tubes containing the sample for 60 sec at 8,200 g at 4 °C - Transfer 30 µl of the supernatant from the "protein" sample (unbound fraction supernatant) into the labeled microfuge tube.
High Throughput Processing of DNA Samples on FTA Paper for PCR Analysis Stanislav Vitha1, David W. Yoder Michigan State University, Department of Plant Biology, 166 Plant .
Supporting applications ranging from clinical and blood banking, microbiology, tissue culture, molecular biology and genomics, drug discovery and proteomics, our centrifuge rotors are designed for maximum application flexibility and best-quality separations. Browse our complete portfolio or let one ...
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